For the pilot DC HTS, a total of 45 pairwise 4×4 DCMs were generated from the 10 test compounds, and these were arrayed onto 3×384-well master plates. In addition to the nine DC wells, each 4×4 DCM contained a DMSO control and three single-drug control wells for each of the two drugs at concentrations tested within the matrix. Source A (40μL) and Source B (20μL) 10× master plates were arrayed manually using a matrix pipettor.
The 384-well transfer head of the Janus MDT Mini platform was used to transfer 20μL from Plate A into Plate B and mix the DC master plates. 20 μL of DMSO was added to single-drug wells, and 40 μL to DMSO control wells. The 384-well transfer head of the Janus MDT Mini was used to transfer 2 μL from the DC master plates into barcoded replica daughter plates, which were then centrifuged at 50×g for 5 min, sealed with aluminum foil, and stored at −20 °C until use.
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Scientific article publishing date: 18/11/2020
Article Identifier BSC22_428EN